显微镜细胞介导细胞毒作用检测法6：
用含10%小牛血清和抗生素的RPMI-1640培养液将K562细胞配成1&&time105/ml浓度。用同样培养液将MC配成1&&time106/ml浓度。2.取96孔圆底细胞培养板，每孔加0.1ml K562细胞悬液;每孔加适量MC...
荧光法检测CM荧光显微镜荧光法检测CMC1.
显微镜细胞介导细胞毒作用检测法6：荧光法检测CMC
1.用含10%小牛和的RPMI-1640培养液将K562细胞配成1&time105/ml浓度。用同样培养液将MC配成1&time106/ml浓度。
2.取96孔圆底，每孔加0.1ml K562细胞悬液;每孔加适量MC使效靶比为1：1～5：1，每种处理3个复孔;3个对照孔只加效应细胞;8个对照孔只加靶细胞;4个对照孔只加细胞培养液。每孔加20l Alamar Blue;每孔总量为0.2ml。
3.在37℃ 5% CO2的饱和水汽二氧化碳培养箱中培养24小时。
4.直接在荧光检测仪上检测荧光强度，激发波长530nm，发射波长590nm。各孔荧光强度值应减去培养液对照荧光强度的平均值，各复孔荧光强度的平均值记为AF。按下式计算特异性杀伤百分比：
BX53TR Research Microscope RMB238000.00
显微镜镜体
BX53TRF Micrscope frame for transmitted and reflected microscopy with built-in filter holders for four filters(LBD, ND6, ND25, an open position for an optional filter of 45mm dia.), including hexagonal driver AB8804, immersion oil 8cc and shield sticker AD4908(2pcs.)Corresponded to WEEE and RoHS regulations 1
中性灰度滤色片
U-25ND25 Neutral density filter for incident light 1
U-25ND6 Neutral density filter for incident light 1
三目镜筒
U-TR30-2 Trinocular tube 1
六孔物镜转盘
U-D6RE Sextuple revolving nosepiece with a slot for analyzer or DICslider 1
机械式载物台
U-SVRB-4 Ceramic surface mechanical stage with right-hand low drivecontrol(long type) 1
夹片器
U-HLDT-4 Specimen holder for BX stage, left hand, for two specimens, thick type 1
100W卤素灯灯室
U-LH100-3 Lamp house for 100W halogen with connecting cable Corresponded to RoHS, WEEE regulations 1
100W汞灯灯室
U-LH100HG Lamp house for 100W mercury burner with collector lens and connecting cable 1
汞灯电源
U-RFL-T Power supply unit for Mercury lamp meeting the RoHS regulation 1
12V100W灯泡
JC12V100WHAL-L Halogen bulb(Philips No. 7724) 2
电源线
UYCP Power cord 2
荧光激发块
U-MWB2 Mirror Unit for fluorescence microscopy, wide band Blue excitation, exciter filter BP450-480, dichroic beamsplitter DM500, barrier filter BA515 1
U-MWG2 Mirror Unit for fluorescence microscopy, wide band Green excitation, exciter filter BP510-550, dichroic beamsplitter DM570, barrier filter BA590 1
荧光反射器
BX-URA2 Universal reflected light illuminator with field stop and aperture stop, including 6-position mirror unit cassette, UV protective shield AD5417, shield plate AB1550, Allen wrench AD6162 and indication plate AD5419 1
防尘罩
COVER-018 Dust cover Type 018 for BX2(L 510mm x W 320mm x H 530 mm) 1
汞灯灯泡
USH103OL 100W mercury burner for U-LH100HGAPO, U-LH100HG 2
目镜
WHN10X Widefield eyepiece 10X 2
无荧光镜油
IMMOIL-F30CC Immersion oil 30cc. Low level auto-fluorescence. 1
相差附件
BX2-PHD-P11 Phase Contrast/Darkfield Attachment 1
相差聚光镜
U-PCD2 Phase contrast dark field condenser NA1.1 1
平场消色差相差物镜
PLN10XPH Plan achromat phase objective 10X/0.25, WD 10.6 1
PLN20XPH Plan achromat phase objective 20X/0.4, WD 1.2(spring) 1
PLN40XPH Plan achromat phase objective 40X/0.65, WD 0.6(spring) 1
PLN100XOPH Plan achromat phase objective 100X/1.25, WD 0.15(spring, oil) 1
对中望远镜
U-CT30 Centering telescope 1
滤色片
43IF550-W45 Interference green contrast filter 45mm dia. 1
简易偏光装置
BX2-POL Simple Polarizing Attachment 1
起偏器
U-POT Polarizer for transmitted light 1
检偏器
U-ANT Analyzer for transmitted light 1
DP72 1200万像素 没必要买DP73
MP5.0-RTV-R-CLR-10-C MicroPublisher 5.0 RTV, 5 mega-pixel CCD Color-Bayer Mosaic, 10-bit, cooled, Real Time Viewing 1 RMB140000
C型视频接口
U-TV0.5XC-3 C-mount video attachment with 0.5X lens 1 RMB8000.00
IPP-60 软件 解密版 5000元
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